The 137 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15,666 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 16kDa by SDS-PAGE. The non-reduced protein
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE
Bio-Activity
The ED50 is 2 - 4 ng/ml, corresponding to a specific activity 5 - 2.5 x 10^5 units/mg, as determined by a dose dependent stimulation of normal human dermal fibroblasts production of IL-6.
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 10 mM NaH2PO4, 300 mM NaCl
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.